Professor Hughes’ PhD research at The University of Queensland focused on developing a symbiotic control strategy of an agricultural disease caused by a viral pathogen transmitted by Planthoppers. To further his expertise in the vector biology and symbiosis fields he undertook a Postdoctoral fellowship at Johns Hopkins School of Public Health, and then a Research Associate position at Penn State University where he examined the interactions between Wolbachia, a common bacterial endosymbiont of insects, other microbiota, and Plasmodium parasites in Anopheles mosquitoes. In 2015, he joined the Department of Pathology at the University of Texas Medical Branch as an Assistant Professor and focused on examining interactions between the microbiome and arboviruses in Aedes mosquitoes. Professor Hughes joined the Departments of Vector Biology and Tropical Disease Biology at LSTM in 2018 where his group works on arboviruses and microbes of mosquitoes.